Hotspot

trnC-GCA-petN

LSC · 28435-29304 · 870 bp

Back to Tabernaemontana

Pi 0.00711

MarkerSeek score 62.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 6
pi: 0.00711
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	961-980 bp	1.0	81.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	959-978 bp	1.0	81.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	962-981 bp	1.0	81.3
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	907-926 bp	1.0	80.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTCTTCCCCACACTACGAGT	50.0	50.0	58.4	58.0	977-996 bp	1.0	79.7