Hotspot

trnK-UUU-rps16

LSC · 4532-5339 · 808 bp

Back to Tabernaemontana

Pi 0.0191
MarkerSeek score 61.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
808
variable_sites
15
pi
0.0191
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAGGGTGGGTGTTTTTAA 55.0 35.0 57.9 53.3 902-923 bp 1.0 45.3
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAAAGGGTGGGTGTTTTTA 55.0 35.0 57.9 53.3 903-924 bp 1.0 45.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAAAGGGTGGGTGTTTTTA 50.0 35.0 58.2 53.3 841-862 bp 1.0 44.8
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAGGGTGGGTGTTTTTAA 50.0 35.0 58.2 53.3 840-861 bp 1.0 44.8
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC AAAAAGGGTGGGTGTTTTTAA 55.0 33.3 57.9 54.2 903-924 bp 1.0 44.6