Hotspot

ndhF

SSC · 114242-116479 · 2238 bp

Back to Syzygium

Pi 0.00728

MarkerSeek score 62.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 325
pi: 0.00728
unique_haplotype_count: 144
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AGAGCCGGTGAATTAGAAAGA	50.0	42.9	58.0	56.7	2316-2997 bp	0.982955	70.6
ndhF_p2	GCAGAATACCGTCACCCATT	AAGAGCCGGTGAATTAGAAAGA	50.0	40.9	58.0	57.4	2317-2998 bp	0.982955	68.3
ndhF_p3	GCAGAATACCGTCACCCATT	AGAGCCGGTGAATTAGAAAGAA	50.0	40.9	58.0	57.4	2316-2997 bp	0.982955	68.3
ndhF_p4	GCAGAATACCGTCACCCATT	GAGCCGGTGAATTAGAAAGA	50.0	45.0	58.0	55.2	2315-2996 bp	0.982955	66.1
ndhF_p5	GCAGAATACCGTCACCCATT	AGAGCCGGTGAATTAGAAAG	50.0	45.0	58.0	55.2	2316-2997 bp	0.960227	65.1