Hotspot

ndhA

SSC · 124033-126202 · 2170 bp

Back to Syzygium

Pi 0.00593

MarkerSeek score 74.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2170
variable_sites: 270
pi: 0.00593
unique_haplotype_count: 154
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGCTATTTGAGAGAAGTCG	CCACCTGGTTTTATCAATTTGCA	39.1	39.1	57.2	58.1	2289-2363 bp	0.965909	50.6
ndhA_p2	GTTTGCTATTTGAGAGAAGTCGT	CCACCTGGTTTTATCAATTTGCA	39.1	39.1	57.2	58.1	2288-2362 bp	0.965909	50.6
ndhA_p3	TGCTATTTGAGAGAAGTCGT	CCACCTGGTTTTATCAATTTGCA	40.0	39.1	54.1	58.1	2285-2359 bp	0.965909	50.6
ndhA_p4	TGTTTGCTATTTGAGAGAAGTCG	TCCACCTGGTTTTATCAATTTGC	39.1	39.1	57.2	57.9	2290-2364 bp	0.965909	50.6
ndhA_p5	GTTTGCTATTTGAGAGAAGTCGT	TCCACCTGGTTTTATCAATTTGC	39.1	39.1	57.2	57.9	2289-2363 bp	0.965909	50.6