Hotspot

petA-psbJ

LSC · 66569-67588 · 1020 bp

Back to Syzygium

Pi 0.00954

MarkerSeek score 73.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 191
pi: 0.00954
unique_haplotype_count: 143
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATACTCCGGGTTGGGTTCAT	50.0	50.0	57.8	58.4	589-1187 bp	1.0	87.5
petA-psbJ_p2	GATCCATTCCGTGTCCAAGG	ATACTCCGGGTTGGGTTCAT	55.0	50.0	58.3	58.4	607-1205 bp	0.994318	85.9
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	TTCATACTCCGGGTTGGGTT	50.0	50.0	57.8	58.6	592-1190 bp	1.0	85.7
petA-psbJ_p4	TGTCCAAGGCCTTTTGTTCT	ATACTCCGGGTTGGGTTCAT	45.0	50.0	57.5	58.4	596-1194 bp	1.0	85.1
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	ATGGTTCATACTCCGGGTTG	50.0	50.0	57.8	57.3	596-1194 bp	0.988636	84.6