Hotspot

rbcL-accD

LSC · 59674-60385 · 712 bp

Back to Syzygium

Pi 0.00562

MarkerSeek score 69.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 108
pi: 0.00562
unique_haplotype_count: 105
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACTTAGCCCACACCTGTATTC	55.0	47.6	58.0	57.4	816-913 bp	1.0	78.7
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTTACTTAGCCCACACCTGT	55.0	45.0	58.0	56.4	819-916 bp	0.994318	78.4
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	ACTTAGCCCACACCTGTATT	55.0	45.0	58.0	56.1	816-913 bp	1.0	76.7
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	ACTTAGCCCACACCTGTATTC	55.0	47.6	57.7	57.4	802-899 bp	0.994318	76.4
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	TTTACTTAGCCCACACCTGT	55.0	45.0	57.7	56.4	805-902 bp	0.988636	76.1