Hotspot

psaA-pafI

LSC · 45104-45867 · 764 bp

Back to Syzygium

Pi 0.00851

MarkerSeek score 67.80

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 122
pi: 0.00851
unique_haplotype_count: 108
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	899-1022 bp	1.0	90.9
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	880-1003 bp	1.0	90.7
psaA-pafI_p3	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	901-1024 bp	0.988636	89.6
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	882-1005 bp	0.988636	89.5
psaA-pafI_p5	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	828-951 bp	0.926136	87.0