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psbZ-trnG-GCC

LSC · 39173-39717 · 545 bp

Back to Syzygium

Pi 0.0188

MarkerSeek score 71.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 135
pi: 0.0188
unique_haplotype_count: 140
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GACGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	444-1066 bp	0.994318	89.3
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	456-1078 bp	1.0	87.4
psbZ-trnG-GCC_p3	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	457-1079 bp	1.0	86.1
psbZ-trnG-GCC_p4	ACGGTTGGTCGAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	443-1065 bp	0.994318	85.6
psbZ-trnG-GCC_p5	GACGGTTGGTCGAGTAACAA	ACTTTAACCTGGGCGGATAG	50.0	50.0	57.9	57.0	479-1101 bp	0.965909	81.5