Hotspot

trnD-GUC-trnY-GUA

LSC · 32819-33275 · 457 bp

Back to Syzygium

Pi 0.0132

MarkerSeek score 68.50

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 74
pi: 0.0132
unique_haplotype_count: 101
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGATTCCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	553-592 bp	1.0	89.9
trnD-GUC-trnY-GUA_p2	ATTTGATTCCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	552-591 bp	1.0	89.9
trnD-GUC-trnY-GUA_p3	ATTTGATTCCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	574-613 bp	1.0	89.0
trnD-GUC-trnY-GUA_p4	ATTTGATTCCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	573-612 bp	1.0	88.8
trnD-GUC-trnY-GUA_p5	ATTTGATTCCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	607-646 bp	0.988636	88.1