Hotspot

psbM-trnD-GUC

LSC · 31649-32744 · 1096 bp

Back to Syzygium

Pi 0.00527

MarkerSeek score 68.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 159
pi: 0.00527
unique_haplotype_count: 128
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	814-1246 bp	1.0	65.0
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	43.5	50.0	58.0	58.6	817-1249 bp	1.0	63.6
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TCAATTGGTTAGAGCACCGC	43.5	50.0	57.9	58.6	821-1253 bp	1.0	62.9
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TCAATTGGTTAGAGCACCGC	40.9	50.0	56.8	58.6	821-1253 bp	1.0	62.3
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TCAATTGGTTAGAGCACCGC	43.5	50.0	58.2	58.6	820-1252 bp	1.0	62.2