Hotspot

trnK-UUU-rps16

LSC · 4476-5044 · 569 bp

Back to Syzygium

Pi 0.00956

MarkerSeek score 59.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 124
pi: 0.00956
unique_haplotype_count: 130
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	704-950 bp	0.994318	91.2
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	705-951 bp	0.994318	91.2
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	566-812 bp	0.988636	91.2
trnK-UUU-rps16_p4	TTCATGGAAAAGCCCTTCCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.1	58.0	853-1101 bp	0.994318	91.1
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	AAGTGTATCGTGCGGGAATC	55.0	50.0	58.0	58.1	1221-1481 bp	0.982955	90.7