Pi
0.0474
MarkerSeek score
68.50
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | CCCCTTTGGTGTCATTGACA | ACGTCGTGATTTTCCCTAGC | 50.0 | 50.0 | 58.0 | 58.0 | 2559-2865 bp | 0.387097 | 65.9 |
| ycf1_p2 | CCCGGAACTTCTTCGTAGTG | ACGTCGTGATTTTCCCTAGC | 55.0 | 50.0 | 58.0 | 58.0 | 2668-2974 bp | 0.387097 | 65.6 |
| ycf1_p3 | CCGGAACTTCTTCGTAGTGG | ACGTCGTGATTTTCCCTAGC | 55.0 | 50.0 | 58.0 | 58.0 | 2667-2973 bp | 0.387097 | 65.6 |
| ycf1_p4 | GAGCTACTGAGGAACAACGG | GAGATCTTCTTCGGCGGATC | 55.0 | 55.0 | 58.0 | 57.9 | 2656-2962 bp | 0.387097 | 65.2 |
| ycf1_p5 | GAGCTACTGAGGAACAACGG | ACGTCGTGATTTTCCCTAGC | 55.0 | 50.0 | 58.0 | 58.0 | 2763-2997 bp | 0.258065 | 59.3 |
| ycf1_p1 | TCTGTGCCAAGGTTTCAGAC | TGAACGGACCATATCGGAGA | 50.0 | 50.0 | 58.0 | 57.9 | 1103-1256 bp | 0.935484 | 88.7 |
| ycf1_p2 | TGATTATGCCTCGCCGAAAA | TGAACGGACCATATCGGAGA | 45.0 | 50.0 | 58.0 | 57.9 | 546-645 bp | 0.83871 | 84.8 |
| ycf1_p3 | TTGATTATGCCTCGCCGAAA | TGAACGGACCATATCGGAGA | 45.0 | 50.0 | 58.0 | 57.9 | 547-646 bp | 0.774194 | 82.2 |
| ycf1_p4 | CTTGCCCATATGGAGTGGTC | TGAACGGACCATATCGGAGA | 55.0 | 50.0 | 58.0 | 57.9 | 834-852 bp | 0.387097 | 56.6 |
| ycf1_p5 | CTTGCCCATATGGAGTGGTC | CATGCCAAAGTGCTGGAAAG | 55.0 | 50.0 | 58.0 | 57.9 | 1128-1146 bp | 0.387097 | 56.1 |