Hotspot

clpP

LSC · 72861-74891 · 2031 bp

Back to Syringa

Pi 0.0295

MarkerSeek score 60.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2031
variable_sites: 201
pi: 0.0295
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TAGCTGACCCTCTTAGTCCG	GCAATGGGGGTTGATACCAT	55.0	50.0	57.9	57.9	2733-2888 bp	0.870968	81.7
clpP_p2	CGTTCTTGACAGAGTGGGAG	GCAATGGGGGTTGATACCAT	55.0	50.0	57.9	57.9	2715-2870 bp	0.870968	81.6
clpP_p3	AGTCGCACATACACCCTAGT	TTCGTTACAGTCCCTCCTGT	50.0	50.0	58.2	58.0	2885-3000 bp	0.290323	53.6
clpP_p4	AGTCGCACATACACCCTAGT	CCCAGCTACACCTTCGTAAC	50.0	55.0	58.2	58.0	2919-2921 bp	0.193548	46.6
clpP_p5	AGTCGCACATACACCCTAGT	CTGCCAAAAAGCATAAGCCC	50.0	50.0	58.2	58.0	2956-2958 bp	0.193548	46.6