Hotspot

trnI-CAU-ycf2

IRb · 88959-89395 · 437 bp

Back to Syringa

Pi 0.0377

MarkerSeek score 60.00

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 437
variable_sites: 55
pi: 0.0377
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	GACCCAACGGATTCTTCCAA	50.0	50.0	58.0	57.8	506-966 bp	1.0	88.5
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	AAGACCCAACGGATTCTTCC	50.0	50.0	58.0	57.5	508-968 bp	1.0	86.1
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	GGAAGACCCAACGGATTCTT	50.0	50.0	58.0	57.5	510-970 bp	1.0	86.1
trnI-CAU-ycf2_p4	AGTTGGGCGCTTTAACCATT	GACCCAACGGATTCTTCCAA	45.0	50.0	58.4	57.8	507-967 bp	1.0	85.6
trnI-CAU-ycf2_p5	TTGGGCGCTTTAACCATTCA	GACCCAACGGATTCTTCCAA	45.0	50.0	58.4	57.8	505-965 bp	1.0	85.5