Hotspot

trnK-UUU-rps16

LSC · 4380-5264 · 885 bp

Back to Syringa

Pi 0.0183
MarkerSeek score 62.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
885
variable_sites
58
pi
0.0183
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATTCCTCGCTTCATCCA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.1 58.2 1045-1062 bp 1.0 85.7
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 997-1014 bp 1.0 85.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 996-1013 bp 1.0 85.2
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1011-1028 bp 1.0 84.3
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1006-1023 bp 1.0 84.2