Hotspot

trnH-GUG-psbA

LSC · 89-519 · 431 bp

Back to Syringa

Pi 0.0489

MarkerSeek score 68.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 78
pi: 0.0489
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GAAGCTCCATCTACAAATGG	50.0	45.0	57.9	53.8	312-518 bp	1.0	57.0
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GAAGCTCCATCTACAAATGG	50.0	45.0	58.2	53.8	313-519 bp	1.0	56.8
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	GAAGCTCCATCTACAAATGG	50.0	45.0	57.7	53.8	305-511 bp	1.0	55.4
trnH-GUG-psbA_p4	GCCTTAATCCACTTGGCTACA	GAAGCTCCATCTACAAATGG	47.6	45.0	58.0	53.8	310-516 bp	1.0	52.0
trnH-GUG-psbA_p5	TGCCTTAATCCACTTGGCTAC	GAAGCTCCATCTACAAATGG	47.6	45.0	58.0	53.8	311-517 bp	1.0	52.0