Hotspot

rps2

LSC · 33720-34472 · 753 bp

Pi 0.168

MarkerSeek score 83.10

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 753
variable_sites: 172
pi: 0.168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GACTAATCCCAGCAGGTTCG	CGTTTGTTGGAGCAGCAAAT	55.0	45.0	58.1	57.9	1415 bp	0.333333	51.8
rps2_p2	GACTAATCCCAGCAGGTTCG	GGAATTAAAGCTCCTGCCCA	55.0	50.0	58.1	57.9	1365 bp	0.333333	51.7
rps2_p3	GACTAATCCCAGCAGGTTCG	AGCTCCTGCCCAATTACAAC	55.0	50.0	58.1	58.2	1357 bp	0.333333	51.6
rps2_p4	GACTAATCCCAGCAGGTTCG	GTCCCATTTGAGTGATGGCA	55.0	50.0	58.1	58.2	1280 bp	0.333333	51.6
rps2_p5	GACTAATCCCAGCAGGTTCG	TATCGTTTGTTGGAGCAGCA	55.0	45.0	58.1	57.8	1418 bp	0.333333	51.4