Hotspot

rpl4

LSC · 82666-83310 · 645 bp

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Pi 0.275
MarkerSeek score 82.90
Primer available yes
Label rpl4

Feature Metadata

label_name
rpl4
feature_type
gene
region
LSC
length_bp
645
variable_sites
234
pi
0.275
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl4_p1 ACGACGTTTATGACCACCAC TCGTGGAGCAATGACTCATG 50.0 50.0 58.2 58.0 1394 bp 0.333333 51.5
rpl4_p2 ACGACGTTTATGACCACCAC GTCGTGGAGCAATGACTCAT 50.0 50.0 58.2 58.0 1395 bp 0.333333 51.5
rpl4_p3 ACGACGTTTATGACCACCAC GTGGAGCAATGACTCATGGT 50.0 50.0 58.2 57.9 1392 bp 0.333333 50.7
rpl4_p4 ACGACGTTTATGACCACCAC GGAACTACTCCAGGACGAGT 50.0 55.0 58.2 58.2 1336 bp 0.333333 50.3
rpl4_p5 GACGTTTATGACCACCACCT TCGTGGAGCAATGACTCATG 50.0 50.0 57.5 58.0 1392 bp 0.333333 49.5