Hotspot

dnaB

LSC · 50414-52186 · 1773 bp

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Pi 0.267
MarkerSeek score 83.00
Primer available yes
Label dnaB

Feature Metadata

label_name
dnaB
feature_type
gene
region
LSC
length_bp
1773
variable_sites
642
pi
0.267
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaB_p1 CCTCTTGAGATTAAGACAGGCC CCTGCGTTCTACCAACTGAA 50.0 50.0 58.2 57.8 2183 bp 0.333333 34.4
dnaB_p2 CCTCTTGAGATTAAGACAGGCC ACCAACTGAACTAAGGACGC 50.0 50.0 58.2 57.8 2173 bp 0.333333 34.4
dnaB_p3 CCTCTTGAGATTAAGACAGGCC AAAAGAACGGATGCAGGAGG 50.0 50.0 58.2 58.2 2518 bp 0.333333 34.4
dnaB_p4 CCTCTTGAGATTAAGACAGGCC ACCCTCGACACCAGATTTTG 50.0 50.0 58.2 57.8 2207 bp 0.333333 34.3
dnaB_p5 CCTCTTGAGATTAAGACAGGCC CAGATTTTGGAGACCTGCGT 50.0 50.0 58.2 58.2 2196 bp 0.333333 34.3