Hotspot

ccs1

IRa · 114831-116201 · 1371 bp

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Pi 0.218
MarkerSeek score 83.50
Primer available yes
Label ccs1

Feature Metadata

label_name
ccs1
feature_type
gene
region
IRa
length_bp
1371
variable_sites
396
pi
0.218
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccs1_p1 ACCTCTTGGTTCCTTGGTTC TAAATGGACGACGCCGAAAA 50.0 45.0 57.3 58.2 2044 bp 0.333333 46.4
ccs1_p2 ACCTCTTGGTTCCTTGGTTC TTAAATGGACGACGCCGAAA 50.0 45.0 57.3 58.2 2045 bp 0.333333 46.4
ccs1_p3 ACCTCTTGGTTCCTTGGTTC TTTAAATGGACGACGCCGAA 50.0 45.0 57.3 58.2 2046 bp 0.333333 46.4
ccs1_p4 CCTCTTGGTTCCTTGGTTCT TAAATGGACGACGCCGAAAA 50.0 45.0 57.0 58.2 2043 bp 0.333333 44.0
ccs1_p5 CCTCTTGGTTCCTTGGTTCT TTAAATGGACGACGCCGAAA 50.0 45.0 57.0 58.2 2044 bp 0.333333 44.0
ccs1_p1 TAAATGGACGACGCCGAAAA ACCTCTTGGTTCCTTGGTTC 45.0 50.0 58.2 57.3 2044 bp 0.333333 46.4
ccs1_p2 TTAAATGGACGACGCCGAAA ACCTCTTGGTTCCTTGGTTC 45.0 50.0 58.2 57.3 2045 bp 0.333333 46.4
ccs1_p3 TTTAAATGGACGACGCCGAA ACCTCTTGGTTCCTTGGTTC 45.0 50.0 58.2 57.3 2046 bp 0.333333 46.4
ccs1_p4 TAAATGGACGACGCCGAAAA CCTCTTGGTTCCTTGGTTCT 45.0 50.0 58.2 57.0 2043 bp 0.333333 44.0
ccs1_p5 TTAAATGGACGACGCCGAAA CCTCTTGGTTCCTTGGTTCT 45.0 50.0 58.2 57.0 2044 bp 0.333333 44.0