Hotspot

rpl27-secA

IRa · 111025-111956 · 932 bp

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Pi 0.325
MarkerSeek score 82.70
Primer available yes
Label rpl27-secA

Feature Metadata

label_name
rpl27-secA
feature_type
igs
region
IRa
length_bp
932
variable_sites
393
pi
0.325
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl27-secA_p1 TCGTGGACATAGACAACCCT TAATGGCGTTCTTGCTTCGT 50.0 45.0 58.1 58.2 1932 bp 0.333333 51.2
rpl27-secA_p2 CGATTGCACAGACTTCAGGA TAATGGCGTTCTTGCTTCGT 50.0 45.0 57.9 58.2 2185 bp 0.333333 51.1
rpl27-secA_p3 TCGTGGACATAGACAACCCT AAACCGGCTAAGATTTGGCT 50.0 45.0 58.1 57.8 1538 bp 0.333333 51.1
rpl27-secA_p4 CGATTGCACAGACTTCAGGA AAACCGGCTAAGATTTGGCT 50.0 45.0 57.9 57.8 1791 bp 0.333333 50.9
rpl27-secA_p5 GGGCAGGAAGTACACGAAAT TAATGGCGTTCTTGCTTCGT 50.0 45.0 57.9 58.2 1807 bp 0.333333 50.9