Hotspot

secA-rpl27

IRb · 106980-107911 · 932 bp

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Pi 0.327
MarkerSeek score 82.70
Primer available yes
Label secA-rpl27

Feature Metadata

label_name
secA-rpl27
feature_type
igs
region
IRb
length_bp
932
variable_sites
396
pi
0.327
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
secA-rpl27_p1 TAATGGCGTTCTTGCTTCGT TCGTGGACATAGACAACCCT 45.0 50.0 58.2 58.1 1932 bp 0.333333 51.2
secA-rpl27_p2 TAATGGCGTTCTTGCTTCGT CGATTGCACAGACTTCAGGA 45.0 50.0 58.2 57.9 2185 bp 0.333333 51.1
secA-rpl27_p3 AAACCGGCTAAGATTTGGCT TCGTGGACATAGACAACCCT 45.0 50.0 57.8 58.1 1538 bp 0.333333 51.1
secA-rpl27_p4 AAACCGGCTAAGATTTGGCT CGATTGCACAGACTTCAGGA 45.0 50.0 57.8 57.9 1791 bp 0.333333 50.9
secA-rpl27_p5 TAATGGCGTTCTTGCTTCGT GGGCAGGAAGTACACGAAAT 45.0 50.0 58.2 57.9 1807 bp 0.333333 50.9