Hotspot

ndhE-psaC

Genome · 107253-107436 · 184 bp

Back to Syntrichia

Pi 0.0652

MarkerSeek score 83.20

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: Genome
length_bp: 184
variable_sites: 12
pi: 0.0652
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TATAGCTATTGCGGCTGCTG	CTTTGCATCCATCCCAAGGT	50.0	50.0	57.9	58.1	80-399 bp	1.0	82.2
ndhE-psaC_p2	TATAGCTATTGCGGCTGCTG	TTGCATCCATCCCAAGGTAC	50.0	50.0	57.9	57.6	80-397 bp	1.0	79.9
ndhE-psaC_p3	TATAGCTATTGCGGCTGCTG	ACATCTGTAGGGCATGCTCT	50.0	50.0	57.9	58.5	80-367 bp	1.0	79.7
ndhE-psaC_p4	TATAGCTATTGCGGCTGCTG	AGCTTTGCATCCATCCCAAG	50.0	50.0	57.9	58.5	80-401 bp	1.0	79.2
ndhE-psaC_p5	TATAGCTATTGCGGCTGCTG	CATCTGTAGGGCATGCTCTT	50.0	50.0	57.9	57.4	80-366 bp	1.0	78.5