Hotspot

ndhG-ndhE

Genome · 106910-106949 · 40 bp

Back to Syntrichia

Pi 0.05

MarkerSeek score 80.20

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: Genome
length_bp: 40
variable_sites: 2
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	AGCATTAATAGGCGCTATTGT	GCTCGAACCATATTTCGGCT	38.1	50.0	55.5	58.4	80-211 bp	1.0	49.9
ndhG-ndhE_p2	AGCATTAATAGGCGCTATTGTT	GCTCGAACCATATTTCGGCT	36.4	50.0	56.3	58.4	80-211 bp	1.0	48.0
ndhG-ndhE_p3	AGCATTAATAGGCGCTATTGT	TGCTCGAACCATATTTCGGC	38.1	50.0	55.5	58.7	80-212 bp	1.0	47.6
ndhG-ndhE_p4	AGCATTAATAGGCGCTATTGTT	TGCTCGAACCATATTTCGGC	36.4	50.0	56.3	58.7	80-212 bp	1.0	45.7
ndhG-ndhE_p5	TGTTATAGCTCGTCGAGAAA	GCTCGAACCATATTTCGGCT	40.0	50.0	54.0	58.4	80-193 bp	1.0	45.7