Hotspot

trnG-GCC-trnR-UCU

Genome · 62628-62701 · 74 bp

Back to Syntrichia

Pi 0.125

MarkerSeek score 76.80

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: Genome
length_bp: 74
variable_sites: 7
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	80-160 bp	1.0	72.0
trnG-GCC-trnR-UCU_p2	CGCCGACTATAACCCTTAGC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.9	56.9	80-171 bp	1.0	71.6
trnG-GCC-trnR-UCU_p3	GCGCCGACTATAACCCTTAG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.9	56.9	80-172 bp	1.0	71.5
trnG-GCC-trnR-UCU_p4	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	80-166 bp	1.0	71.2
trnG-GCC-trnR-UCU_p5	CGCCGACTATAACCCTTAGC	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	57.9	57.9	80-177 bp	1.0	70.8