Hotspot

trnE-UUC-trnY-GUA

Genome · 46147-46239 · 93 bp

Back to Syntrichia

Pi 0.111

MarkerSeek score 85.10

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 93
variable_sites: 10
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	CTCTCTTTCAAGGAGGCGAC	ATTGCCAGCGGATTTACAGT	55.0	45.0	58.0	57.9	189-191 bp	1.0	84.4
trnE-UUC-trnY-GUA_p2	ATCGTCTAGTGGCCTAGGAC	ATTGCCAGCGGATTTACAGT	55.0	45.0	58.0	57.9	211-213 bp	1.0	83.7
trnE-UUC-trnY-GUA_p3	CATCGTCTAGTGGCCTAGGA	ATTGCCAGCGGATTTACAGT	55.0	45.0	58.0	57.9	212-214 bp	1.0	83.7
trnE-UUC-trnY-GUA_p4	CTCTCTTTCAAGGAGGCGAC	GAAAATTGGGTCGAGCTGGA	55.0	50.0	58.0	58.2	226-228 bp	1.0	83.1
trnE-UUC-trnY-GUA_p5	ATCGTCTAGTGGCCTAGGAC	GAAAATTGGGTCGAGCTGGA	55.0	50.0	58.0	58.2	248-250 bp	1.0	82.6