Hotspot

trnfM-CAU-trnG-GCC

Genome · 41777-41950 · 174 bp

Back to Syntrichia

Pi 0.0471

MarkerSeek score 70.90

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 174
variable_sites: 8
pi: 0.0471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	GTAGCTCGCAAGGCTCATAA	AATATGCGGGTTCGATTCCC	50.0	50.0	57.8	58.1	259-262 bp	1.0	81.0
trnfM-CAU-trnG-GCC_p2	TAGCTCGCAAGGCTCATAAC	AATATGCGGGTTCGATTCCC	50.0	50.0	57.8	58.1	258-261 bp	1.0	81.0
trnfM-CAU-trnG-GCC_p3	GTAGCTCGCAAGGCTCATAA	AAGGAGAATATGCGGGTTCG	50.0	50.0	57.8	57.8	265-268 bp	1.0	79.9
trnfM-CAU-trnG-GCC_p4	TAGCTCGCAAGGCTCATAAC	AAGGAGAATATGCGGGTTCG	50.0	50.0	57.8	57.8	264-267 bp	1.0	79.9
trnfM-CAU-trnG-GCC_p5	TAGCTCGCAAGGCTCATAAC	TTGCCAAGGAGAATATGCGG	50.0	50.0	57.8	58.3	269-272 bp	1.0	79.2