Hotspot

psbJ-orf40

Genome · 19152-19421 · 270 bp

Back to Syntrichia

Pi 0.0448

MarkerSeek score 73.10

Primer available yes

Label psbJ-orf40

Feature Metadata

label_name: psbJ-orf40
feature_type: igs
region: Genome
length_bp: 270
variable_sites: 12
pi: 0.0448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-orf40_p1	AGGTACTGTAGCCGGTATTCT	TGCACTTTTTAAAATGGCTCGT	47.6	36.4	57.7	57.7	422-424 bp	1.0	53.5
psbJ-orf40_p2	GGCTCCTATTCTGGATTAGGC	TGCACTTTTTAAAATGGCTCGT	52.4	36.4	57.7	57.7	370-372 bp	1.0	53.5
psbJ-orf40_p3	GCCGGTATTCTTGTAATTGGC	TGCACTTTTTAAAATGGCTCGT	47.6	36.4	57.7	57.7	412-414 bp	1.0	53.2
psbJ-orf40_p4	ACTGTAGCCGGTATTCTTGT	TGCACTTTTTAAAATGGCTCGT	45.0	36.4	56.3	57.7	418-420 bp	1.0	50.1
psbJ-orf40_p5	GGTACTGTAGCCGGTATTCT	TGCACTTTTTAAAATGGCTCGT	50.0	36.4	56.1	57.7	421-423 bp	1.0	49.1