Hotspot

psaJ-trnP-UGG

Genome · 16651-17048 · 398 bp

Back to Syntrichia

Pi 0.0328

MarkerSeek score 66.90

Primer available yes

Label psaJ-trnP-UGG

Feature Metadata

label_name: psaJ-trnP-UGG
feature_type: igs
region: Genome
length_bp: 398
variable_sites: 13
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP-UGG_p1	GTAGCTAATACGGGTGCTGT	TACCAAACTGCGCTACATCC	50.0	50.0	57.1	58.0	467 bp	1.0	75.3
psaJ-trnP-UGG_p2	GTAGCTAATACGGGTGCTGT	GAACCTGCGGCATTTTGTAC	50.0	50.0	57.1	58.0	502 bp	1.0	75.1
psaJ-trnP-UGG_p3	GTAGCTAATACGGGTGCTGT	GGGATGACAGGATTCGAACC	50.0	55.0	57.1	58.0	517 bp	1.0	74.9
psaJ-trnP-UGG_p4	GTAGCTAATACGGGTGCTGT	AAACAAACGCGCTACCAAAC	50.0	45.0	57.1	58.2	479 bp	1.0	74.1
psaJ-trnP-UGG_p5	GTAGCTAATACGGGTGCTGT	CAAAACAAACGCGCTACCAA	50.0	45.0	57.1	58.2	481 bp	1.0	74.0