Hotspot

clpP-rps12

Genome · 14357-14498 · 142 bp

Back to Syntrichia

Pi 0.0725

MarkerSeek score 73.30

Primer available yes

Label clpP-rps12

Feature Metadata

label_name: clpP-rps12
feature_type: igs
region: Genome
length_bp: 142
variable_sites: 10
pi: 0.0725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rps12_p1	GCTGTTGCTATAGAAAACACCT	TCAATTGTTGAATAGTAGGCA	40.9	33.3	56.7	52.8	184-188 bp	1.0	46.0
clpP-rps12_p2	GCTGTTGCTATAGAAAACACCT	ATCAATTGTTGAATAGTAGGCA	40.9	31.8	56.7	53.5	185-189 bp	1.0	46.0
clpP-rps12_p3	GCTGTTGCTATAGAAAACACCT	TCAATTGTTGAATAGTAGGCAT	40.9	31.8	56.7	53.5	184-188 bp	1.0	46.0
clpP-rps12_p4	GCTGTTGCTATAGAAAACACC	TCAATTGTTGAATAGTAGGCA	42.9	33.3	55.2	52.8	184-188 bp	1.0	46.0
clpP-rps12_p5	GCTGTTGCTATAGAAAACACCT	AATCAATTGTTGAATAGTAGGCA	40.9	30.4	56.7	54.3	186-190 bp	1.0	46.0