Hotspot

trnI-CAU-rpl23

Genome · 136-357 · 222 bp

Back to Syntrichia

Pi 0.0676

MarkerSeek score 72.00

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: Genome
length_bp: 222
variable_sites: 15
pi: 0.0676
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	TGAACGGTTAAAGCACCCAA	ACGGGGTATTTTACTTCATCCA	45.0	40.9	57.9	57.2	308-309 bp	1.0	60.7
trnI-CAU-rpl23_p2	AAGCATCCATAGCTGAACGG	ACGGGGTATTTTACTTCATCCA	50.0	40.9	58.0	57.2	321-322 bp	1.0	60.6
trnI-CAU-rpl23_p3	AACGGTTAAAGCACCCAACT	ACGGGGTATTTTACTTCATCCA	45.0	40.9	57.9	57.2	306-307 bp	1.0	60.6
trnI-CAU-rpl23_p4	GCATCCATAGCTGAACGGTT	ACGGGGTATTTTACTTCATCCA	50.0	40.9	58.3	57.2	319-320 bp	1.0	58.3
trnI-CAU-rpl23_p5	ACGGTTAAAGCACCCAACTC	ACGGGGTATTTTACTTCATCCA	50.0	40.9	58.4	57.2	305-306 bp	1.0	58.0