Hotspot

ycf4

LSC · 59565-60119 · 555 bp

Back to Synotis

Pi 0.0036

MarkerSeek score 32.80

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 3
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GATGCGGGTTACTTCGGAAA	TTGCCCTTCTGCTGAGAAAG	50.0	50.0	58.3	58.1	682 bp	1.0	77.4
ycf4_p2	GATGCGGGTTACTTCGGAAA	TGATTCTTGCGTTTTTGCCC	50.0	45.0	58.3	57.9	696 bp	1.0	77.0
ycf4_p3	ACTTCGGAAACAAACGGACT	TTGCCCTTCTGCTGAGAAAG	45.0	50.0	57.7	58.1	672 bp	1.0	77.0
ycf4_p4	ACTTCGGAAACAAACGGACT	TGATTCTTGCGTTTTTGCCC	45.0	45.0	57.7	57.9	686 bp	1.0	76.6
ycf4_p5	GATGCGGGTTACTTCGGAAA	TTCTTGCGTTTTTGCCCTTC	50.0	45.0	58.3	57.8	693 bp	1.0	76.4