Hotspot

rpoC1

LSC · 16240-19047 · 2808 bp

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Pi 0.00119

MarkerSeek score 42.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2808
variable_sites: 6
pi: 0.00119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCTGAGAAGACTTTCCAGCT	TTCTGCCATCAGTACCTCCA	45.0	50.0	55.8	58.0	2981-2995 bp	1.0	62.6
rpoC1_p2	TCTGAGAAGACTTTCCAGCT	CGTTCTGCCATCAGTACCTC	45.0	55.0	55.8	58.1	2983-2997 bp	1.0	62.4
rpoC1_p3	TCTGAGAAGACTTTCCAGCT	TCTGCCATCAGTACCTCCAT	45.0	50.0	55.8	57.8	2980-2994 bp	1.0	61.5
rpoC1_p4	TCTGAGAAGACTTTCCAGCTT	TTCTGCCATCAGTACCTCCA	42.9	50.0	56.6	58.0	2981-2995 bp	1.0	60.9
rpoC1_p5	TCCAGCTTAATAGGAAGGAAGC	TTCTGCCATCAGTACCTCCA	45.5	50.0	57.6	58.0	2968-2982 bp	1.0	60.8