Hotspot

ndhF

SSC · 108256-110481 · 2226 bp

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Pi 0.00285

MarkerSeek score 58.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 10
pi: 0.00285
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTTTCGATGCAACAGCAAG	TCGAAAGAGGTAAAATCCGGT	45.0	42.9	57.6	57.0	2394-2395 bp	1.0	61.4
ndhF_p2	TGTTTCGATGCAACAGCAAG	CGAAAGAGGTAAAATCCGGTG	45.0	47.6	57.6	57.0	2393-2394 bp	1.0	61.0
ndhF_p3	TGTTTCGATGCAACAGCAAG	AGAGGTAAAATCCGGTGAATCG	45.0	45.5	57.6	58.2	2389-2390 bp	1.0	59.4
ndhF_p4	TGTTTCGATGCAACAGCAAG	GAGGTAAAATCCGGTGAATCG	45.0	47.6	57.6	56.8	2388-2389 bp	1.0	59.4
ndhF_p5	GTTTCGATGCAACAGCAAGA	TCGAAAGAGGTAAAATCCGGT	45.0	42.9	57.3	57.0	2393-2394 bp	1.0	59.1