Hotspot

rps15-ycf1

SSC · 121787-121998 · 212 bp

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Pi 0.0102

MarkerSeek score 55.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 212
variable_sites: 4
pi: 0.0102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCTCCGTATCTTATTGGTGA	GGGAGCCGTTTGAGTATGTT	40.9	50.0	56.1	57.9	358-388 bp	1.0	50.7
rps15-ycf1_p2	AGTCTCCGTATCTTATTGGTGA	ATGGGAGCCGTTTGAGTATG	40.9	50.0	56.1	57.7	360-390 bp	1.0	48.9
rps15-ycf1_p3	AAGTCTCCGTATCTTATTGGTGA	GGGAGCCGTTTGAGTATGTT	39.1	50.0	56.8	57.9	359-389 bp	1.0	48.4
rps15-ycf1_p4	AGTCTCCGTATCTTATTGGTGAA	GGGAGCCGTTTGAGTATGTT	39.1	50.0	56.8	57.9	358-388 bp	1.0	48.4
rps15-ycf1_p5	AGTCTCCGTATCTTATTGGTGAA	ATGGGAGCCGTTTGAGTATG	39.1	50.0	56.8	57.7	360-390 bp	1.0	46.6