Hotspot

ndhF-rpl32

SSC · 110482-111547 · 1066 bp

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Pi 0.0036
MarkerSeek score 38.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1066
variable_sites
7
pi
0.0036
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGCCCACACGATTAGTTACA CTTATATCTGCCCCTCCCGA 50.0 55.0 58.0 58.1 2061-2071 bp 1.0 80.5
ndhF-rpl32_p2 CGCCCACACGATTAGTTACA TCTTATATCTGCCCCTCCCG 50.0 55.0 58.0 58.1 2062-2072 bp 1.0 80.5
ndhF-rpl32_p3 CGCCCACACGATTAGTTACA TTATATCTGCCCCTCCCGAG 50.0 55.0 58.0 58.1 2060-2070 bp 1.0 80.5
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG TTATATCTGCCCCTCCCGAG 50.0 55.0 58.0 58.1 1602-1612 bp 1.0 80.5
ndhF-rpl32_p5 CGCCCACACGATTAGTTACA AAAAAGACTCCCCACCACAC 50.0 50.0 58.0 57.9 1998-2010 bp 1.0 80.3