Hotspot

ndhC-trnV-UAC

LSC · 50437-51202 · 766 bp

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Pi 0.00435

MarkerSeek score 53.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 6
pi: 0.00435
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCTTTGCTAATCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.1	57.8	903-911 bp	1.0	72.4
ndhC-trnV-UAC_p2	TCGGAGCTAAAAATCCCGAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	56.9	57.8	892-900 bp	1.0	71.2
ndhC-trnV-UAC_p3	CCCTTTGCTAATCGGAGCTAA	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.8	57.8	903-911 bp	1.0	70.1
ndhC-trnV-UAC_p4	CCCTTTGCTAATCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.1	57.5	902-910 bp	1.0	69.6
ndhC-trnV-UAC_p5	GGCCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	57.9	50.0	58.3	57.8	905-913 bp	1.0	69.3