Hotspot

rps14-psaB

LSC · 36899-37865 · 967 bp

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Pi 0.00604

MarkerSeek score 57.70

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 11
pi: 0.00604
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGCCTCTTCTTTTCCCTCTG	CTTTGGTACTCCGGAGAAGC	50.0	55.0	57.1	58.0	1045-1114 bp	1.0	77.8
rps14-psaB_p2	TGCCTCTTCTTTTCCCTCTG	ATGCTTGCCTTTGGTACTCC	50.0	50.0	57.1	58.2	1053-1122 bp	1.0	76.6
rps14-psaB_p3	TGCCTCTTCTTTTCCCTCTG	TGTCATGCTTGCCTTTGGTA	50.0	45.0	57.1	57.7	1057-1126 bp	1.0	75.6
rps14-psaB_p4	TGCCTCTTCTTTTCCCTCTG	ATATGGACTCCGGAGAAGCA	50.0	50.0	57.1	57.9	1036 bp	0.25	42.4
rps14-psaB_p5	TGCCTCTTCTTTTCCCTCTG	TATGGACTCCGGAGAAGCAA	50.0	50.0	57.1	58.1	1035 bp	0.25	41.9