Hotspot

rps16-trnQ-UUG

LSC · 6320-7272 · 953 bp

Back to Synotis

Pi 0.00175

MarkerSeek score 56.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 3
pi: 0.00175
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1026-1048 bp	1.0	78.5
rps16-trnQ-UUG_p2	AGAATAAGAATCCACGCCGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1058-1080 bp	1.0	77.7
rps16-trnQ-UUG_p3	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1041-1063 bp	1.0	76.7
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1040-1062 bp	1.0	76.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1030-1052 bp	1.0	73.8