Hotspot

rpl16

LSC · 87092-89075 · 1984 bp

Back to Syngonium

Pi 0.00471

MarkerSeek score 56.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1984
variable_sites: 9
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCCCTTTTCTTTTGTTCTACA	TCCTCCTGTTTGTTGAATCA	36.4	40.0	56.4	54.5	2037-2100 bp	1.0	43.2
rpl16_p2	TGCCCTTTTCTTTTGTTCTACA	TCCTCCTGTTTGTTGAATCAA	36.4	38.1	56.4	55.3	2037-2100 bp	1.0	43.2
rpl16_p3	TGCCCTTTTCTTTTGTTCTACA	TTCCTCCTGTTTGTTGAATCA	36.4	38.1	56.4	55.3	2038-2101 bp	1.0	43.2
rpl16_p4	TCCTAATGCCCTTTTCTTTTGT	TCCTCCTGTTTGTTGAATCA	36.4	40.0	56.1	54.5	2043-2106 bp	1.0	43.2
rpl16_p5	TCATTCATTCCTAATGCCCT	TCCTCCTGTTTGTTGAATCA	40.0	40.0	54.0	54.5	2051-2114 bp	1.0	43.2