Hotspot

ndhB-tRNA-Leu(CAA)

IRa · 153634-154785 · 1152 bp

Back to Syngonium

Pi 0.00189

MarkerSeek score 63.80

Primer available yes

Label ndhB-tRNA-Leu(CAA)

Feature Metadata

label_name: ndhB-tRNA-Leu(CAA)
feature_type: igs
region: IRa
length_bp: 1152
variable_sites: 2
pi: 0.00189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-tRNA-Leu(CAA)_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	1140-1212 bp	1.0	83.6
ndhB-tRNA-Leu(CAA)_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	1138-1210 bp	1.0	83.6
ndhB-tRNA-Leu(CAA)_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	1135-1207 bp	1.0	82.1
ndhB-tRNA-Leu(CAA)_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	1139-1211 bp	1.0	81.5
ndhB-tRNA-Leu(CAA)_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	1137-1209 bp	1.0	81.3