Hotspot

tRNA-Asn(GUU)-tRNA-Arg(ACG)

IRa · 139459-140043 · 585 bp

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Pi 0.00518

MarkerSeek score 62.40

Primer available yes

Label tRNA-Asn(GUU)-tRNA-Arg(ACG)

Feature Metadata

label_name: tRNA-Asn(GUU)-tRNA-Arg(ACG)
feature_type: igs
region: IRa
length_bp: 585
variable_sites: 3
pi: 0.00518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn(GUU)-tRNA-Arg(ACG)_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	720-726 bp	1.0	79.2
tRNA-Asn(GUU)-tRNA-Arg(ACG)_p2	CACCCGTTGTTCCTCAGTAG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.9	57.8	723-729 bp	1.0	78.6
tRNA-Asn(GUU)-tRNA-Arg(ACG)_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	671-677 bp	1.0	78.1
tRNA-Asn(GUU)-tRNA-Arg(ACG)_p4	GAATCACCCGTTGTTCCTCA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	727-733 bp	1.0	77.8
tRNA-Asn(GUU)-tRNA-Arg(ACG)_p5	AATCACCCGTTGTTCCTCAG	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	726-732 bp	1.0	77.8