Hotspot

petA-psbJ

LSC · 68567-69648 · 1082 bp

Back to Syngonium

Pi 0.00608

MarkerSeek score 66.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1082
variable_sites: 6
pi: 0.00608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCGTTACGTGTTCAAGGTCT	TCCGGATTAGGCTCATCTCT	50.0	50.0	57.9	57.3	1130-1223 bp	1.0	77.8
petA-psbJ_p2	CCGTTACGTGTTCAAGGTCT	CCGGATTAGGCTCATCTCTG	50.0	55.0	57.9	57.3	1129-1222 bp	1.0	77.3
petA-psbJ_p3	CCGTTACGTGTTCAAGGTCT	TTCCGGATTAGGCTCATCTCT	50.0	47.6	57.9	58.0	1131-1224 bp	1.0	75.0
petA-psbJ_p4	CCGTTACGTGTTCAAGGTCT	CCTGTGATCGGTTCAATTGG	50.0	50.0	57.9	56.8	1172-1265 bp	1.0	73.5
petA-psbJ_p5	CCGTTACGTGTTCAAGGTCT	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.9	56.7	1180-1273 bp	1.0	73.0