Hotspot

tRNA-Glu(UUC)-tRNA-Thr(GGU)

LSC · 33818-34693 · 876 bp

Back to Syngonium

Pi 0.00799

MarkerSeek score 63.30

Primer available yes

Label tRNA-Glu(UUC)-tRNA-Thr(GGU)

Feature Metadata

label_name: tRNA-Glu(UUC)-tRNA-Thr(GGU)
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 7
pi: 0.00799
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	963-1007 bp	1.0	79.4
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	961-1005 bp	1.0	79.4
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	942-986 bp	1.0	78.9
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	940-984 bp	1.0	78.9
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	960-1004 bp	1.0	78.8