Hotspot

tRNA-Lys(UUU)-rps16

LSC · 4484-5273 · 790 bp

Back to Syngonium

Pi 0.00257
MarkerSeek score 61.80
Primer available yes
Label tRNA-Lys(UUU)-rps16

Feature Metadata

label_name
tRNA-Lys(UUU)-rps16
feature_type
igs
region
LSC
length_bp
790
variable_sites
2
pi
0.00257
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys(UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 872-883 bp 1.0 79.8
tRNA-Lys(UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 871-882 bp 1.0 79.8
tRNA-Lys(UUU)-rps16_p3 CCGCACTTAAAAGCCGAGTA AGCTCAACCCACAAGAACTG 50.0 50.0 58.3 58.0 881-892 bp 1.0 78.9
tRNA-Lys(UUU)-rps16_p4 AAAGCCGAGTACTCTACCGT GAGCTCAACCCACAAGAACT 50.0 50.0 58.2 57.7 873-884 bp 1.0 77.9
tRNA-Lys(UUU)-rps16_p5 AAGCCGAGTACTCTACCGTT GAGCTCAACCCACAAGAACT 50.0 50.0 58.2 57.7 872-883 bp 1.0 77.9