Hotspot

rpl16

LSC · 86274-87670 · 1397 bp

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Pi 0.00107

MarkerSeek score 59.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 3
pi: 0.00107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTGCTCTTTCAATGCAAAGGG	GGGTTGAATAAAAATTCGATTGACC	47.6	36.0	58.0	57.5	1542-1554 bp	1.0	40.4
rpl16_p2	TGCTCTTTCAATGCAAAGGG	GGGTTGAATAAAAATTCGATTGACC	45.0	36.0	56.9	57.5	1541-1553 bp	1.0	40.4
rpl16_p3	CTGCTCTTTCAATGCAAAGGG	AAATTCGATTGACCATTTGG	47.6	35.0	58.0	52.4	1531-1543 bp	1.0	40.4
rpl16_p4	GCAAAGGGCAAAGGAAAAAGA	GGGTTGAATAAAAATTCGATTGACC	42.9	36.0	57.8	57.5	1529-1541 bp	1.0	40.4
rpl16_p5	CTGCTCTTTCAATGCAAAGGG	AAAATTCGATTGACCATTTGG	47.6	33.3	58.0	53.3	1532-1544 bp	1.0	40.4