Hotspot

petD

LSC · 81627-82838 · 1212 bp

Back to Syndiclis

Pi 0.000825

MarkerSeek score 59.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1212
variable_sites: 2
pi: 0.000825
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTGCATTTTCCGAAGAGAAGA	ACGCGTACTATTTCACGGTT	40.9	45.0	58.0	57.6	1269-1274 bp	1.0	61.6
petD_p2	AGTTGAAGTGCATTTTCCGA	ACGCGTACTATTTCACGGTT	40.0	45.0	55.9	57.6	1275-1280 bp	1.0	60.6
petD_p3	TGAAGTGCATTTTCCGAAGAG	ACGCGTACTATTTCACGGTT	42.9	45.0	56.8	57.6	1272-1277 bp	1.0	60.2
petD_p4	GTTGAAGTGCATTTTCCGAAGA	ACGCGTACTATTTCACGGTT	40.9	45.0	57.8	57.6	1274-1279 bp	1.0	59.8
petD_p5	AGTTGAAGTGCATTTTCCGAAG	ACGCGTACTATTTCACGGTT	40.9	45.0	57.8	57.6	1275-1280 bp	1.0	59.8