Hotspot

matK

LSC · 3698-5221 · 1524 bp

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Pi 0.00262

MarkerSeek score 60.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 8
pi: 0.00262
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCACTACACTACTGCATGAGC	CTTGTTCTGACCGTATCGCA	52.4	50.0	58.5	58.0	1715 bp	1.0	68.7
matK_p2	CCACTACACTACTGCATGAGC	TTGTTCTGACCGTATCGCAC	52.4	50.0	58.5	58.3	1714 bp	1.0	66.4
matK_p3	TCAACCACTACACTACTGCA	CTTGTTCTGACCGTATCGCA	45.0	50.0	56.1	58.0	1719 bp	1.0	65.3
matK_p4	ACCACTACACTACTGCATGAG	CTTGTTCTGACCGTATCGCA	47.6	50.0	57.1	58.0	1716 bp	1.0	65.3
matK_p5	CACTACACTACTGCATGAGC	CTTGTTCTGACCGTATCGCA	50.0	50.0	56.0	58.0	1714 bp	1.0	64.1