Hotspot

ccsA

SSC · 120407-121357 · 951 bp

Back to Syndiclis

Pi 0.0014

MarkerSeek score 45.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 2
pi: 0.0014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GATTGATATGCCTGGCCCAA	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	2009-2012 bp	1.0	80.7
ccsA_p2	GATTGATATGCCTGGCCCAA	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	2217-2220 bp	1.0	80.1
ccsA_p3	TATGCCTGGCCCAATACCTA	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	2003-2006 bp	1.0	79.7
ccsA_p4	TAGACACGCTGCTCTTAGGA	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1483-1485 bp	1.0	79.5
ccsA_p5	GATTGATATGCCTGGCCCAA	CATTGCCAGGAATGAGTGGT	50.0	50.0	58.0	58.2	2214-2217 bp	1.0	79.5