Hotspot

rpl32-trnL-UAG

SSC · 118861-120219 · 1359 bp

Back to Syndiclis

Pi 0.00368

MarkerSeek score 60.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1359
variable_sites: 10
pi: 0.00368
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGAAAGGGATGTCAGGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	1490-1495 bp	1.0	78.2
rpl32-trnL-UAG_p2	AAAGGAAAGGGATGTCAGGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	1483-1488 bp	1.0	77.7
rpl32-trnL-UAG_p3	AAAGGAAAGGGATGTCAGGC	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.8	57.8	1489-1494 bp	1.0	77.6
rpl32-trnL-UAG_p4	AAAGGAAAGGGATGTCAGGC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.8	57.8	1488-1493 bp	1.0	77.6
rpl32-trnL-UAG_p5	AAAGGAAAGGGATGTCAGGC	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.8	58.3	1484-1489 bp	1.0	77.0